Two color RNA intercalating probe for cell imaging applications.

نویسندگان

  • Nathan Stevens
  • Naphtali O'Connor
  • Harshad Vishwasrao
  • Diana Samaroo
  • Eric R Kandel
  • Daniel L Akins
  • Charles M Drain
  • Nicholas J Turro
چکیده

Here we report on a phenanthridine derivative which has a covalently linked fluorescein molecule in order to increase the light absorption and hence fluorescence signal intensity when bound to duplex RNA. Steady-state fluorescence shows that the energy transfer efficiency from the fluorescein to the phenanthridine fluorophore is approximately 77%, which results in the probe being over 5x brighter than other phenanthridine derivatives when bound to RNA. Due to the relatively long lifetime (approximately 20 ns) of the probe, time-resolved fluorescence is used to increase the signal to background ratio in cell growth medium from 7 (steady-state value) to over 40. Moreover, fluorescence images of cells containing the probe show that the fluorescein signal is readily apparent along with that of the intercalated fluorophore, allowing this probe to be used as a dual color probe which simultaneously reports the probes' location and that of RNA.

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عنوان ژورنال:
  • Journal of the American Chemical Society

دوره 130 23  شماره 

صفحات  -

تاریخ انتشار 2008